TLT 1001 - Temel Laboratuvar Uygulamaları I

Basic Information

Course Code:

TLT 1001

Course Name:

Temel Laboratuvar Uygulamaları I

Language of Instruction:

Turkish

Course Type:

Class

Course Level:

Pre Bachelor

ECTS:

6.00

Instructor:

Dr. Öğr. Üyesi Sezgin AKSAKAL CESUR

Course Objectives

To gain skills in laboratory safety, equipment usage, basic solution preparation, sample collection/acceptance criteria, and basic microscopic examination

Course Content

This course begins with laboratory biosafety rules, the use of personal protective equipment, and the principles of medical waste management. Proper operation, maintenance, and sterilization/disinfection methods for primary and auxiliary laboratory equipment (micropipettes, centrifuges, incubators, autoclaves, etc.) are covered through hands-on practice. Following the introduction of the operating principles and use of the light microscope, practical applications are carried out on chemical calculations, preparation of solutions at desired concentrations, serial dilutions, and pH measurement techniques. The course concludes with venipuncture and capillary blood collection techniques, sample acceptance and rejection criteria, smear preparation, basic staining methods (simple staining and Gram staining), and the evaluation of pre-analytical sources of error.

Prerequisites / Corequisites

None.

Learning Outcomes

Code	Description
LO1	Learns theoretical and practical information about the field at a basic level.
LO2	Have a good command of the use of laboratory equipment and routine laboratory procedures.
LO3	Have knowledge and experience in subjects such as occupational safety, laboratory safety, environmental protection.
LO4	Explains basic analytical methods applied in the laboratory and their theoretical principles.
LO5	Fulfills the assigned tasks with a sense of responsibility by using the theoretical knowledge he has.

Weekly Course Content

Week	Content
1	Laboratory Orientation and Biosafety
2	Introduction and Use of Basic Laboratory Equipment
3	Introduction to Glassware, Cleaning, and Sterilization Methods
4	Light Microscope Use and Maintenance
5	Chemical Calculations and Solution Preparation - I
6	Chemical Calculations and Solution Preparation - II
7	pH Measurement and Buffer Solutions
8	Midterm Exam
9	Biological Material Collection and Preparation I
10	Biological Material Collection and Preparation II
11	Basic Dyeing Techniques - I (Simple Staining and Spreading Preparation)
12	Basic Dyeing Techniques - II (Gram Staining )
13	Quality Control and Preanalytical Errors in the Laboratory I
14	Quality Control and Preanalytical Errors in the Laboratory II

Workload Calculation

No information available.

Assessment

Assessment information for this course has not been entered for the current term yet.

PO - LO Matrix

PO \ LO	LO1	LO2	LO3	LO4	LO5
PO-1
PO-2
PO-3
PO-4
PO-5
PO-6
PO-7
PO-8
PO-9
PO-10
PO-11
PO-12
PO-13
PO-14
PO-15

1

Low Contribution

2

Medium Contribution

3

High Contribution